Journal: Molecules

Article Title: Myrtus Polyphenols, from Antioxidants to Anti-Inflammatory Molecules: Exploring a Network Involving Cytochromes P450 and Vitamin D

doi: 10.3390/molecules24081515

Figure Lengend Snippet: Figure 1. ROS levels after oxidative stress. The reactive oxygen species (ROS) production was evaluated in HFF1 exposed for 12 h (Panel A) or 24 h (Panel B) to ascorbic acid (AA, orange bar) or to 0.5, 0.75 and 1 mg/mL seeds (blue bars), or to 0.5, 0.75 and 1 mg/mL pulp (yellow bars) waste extracts, then induced to oxidative stress. “H2O2” (grey bar) represents HFF1 cells exposed to H2O2 alone, without a previous extracts-treatment. ROS levels of treated cells are expressed as a percentage of control untreated HFF1 (Ctrl, black bar), considered as 1. The concentrations were read as the

Article Snippet: Human skin fibroblast 1 (HFF1) cells were purchased from (ATCC, Manassas, VA, USA) and cultured in a basic growing medium of DMEM low glucose (Life Technologies, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (FBS Life Technologies, Carlsbad, California, USA), 2 mM L-glutamine (Euroclone, Milano, Italy) and 1% of penicillin/streptomycin (Euroclone, Milano, Italy).

Techniques: Control

Journal: Molecules

Article Title: Myrtus Polyphenols, from Antioxidants to Anti-Inflammatory Molecules: Exploring a Network Involving Cytochromes P450 and Vitamin D

doi: 10.3390/molecules24081515

Figure Lengend Snippet: Figure 2. Gene expression of proinflammatory cytokines IL-1β and TNF-α. The expression of Interleukin 1 beta (IL-1β) and Tumor necrosis factor alpha (TNF-α) was evaluated in cells pre-treated with the extracts for 12 h and 24 h and then exposed to H2O2 (Panels A and B, and panels C and D, respectively). HFF1 were exposed to ascorbic acid (AA, orange bar), or to 0.5, 0.75 and 1 mg/mL seeds waste extracts (blue bars) or 0.5, 0.75 and 1 mg/ml pulp waste extracts (yellows bar). H2O2 (grey bar) represents HFF1 cells exposed to only H2O2, without previous extracts treatment. The mRNA levels for each gene was expressed as fold of change (2−∆∆Ct) of mRNA levels observed in untreated HFF1 (CTRL, black bar) defined as 1 (mean ±SD; n = 6) and normalized to Glyceraldehyde-3-Phosphate- Dehidrogenase (GAPDH). Data are represented as mean ± SD referred to the control (* p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001).

Article Snippet: Human skin fibroblast 1 (HFF1) cells were purchased from (ATCC, Manassas, VA, USA) and cultured in a basic growing medium of DMEM low glucose (Life Technologies, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (FBS Life Technologies, Carlsbad, California, USA), 2 mM L-glutamine (Euroclone, Milano, Italy) and 1% of penicillin/streptomycin (Euroclone, Milano, Italy).

Techniques: Gene Expression, Expressing, Control

Journal: Molecules

Article Title: Myrtus Polyphenols, from Antioxidants to Anti-Inflammatory Molecules: Exploring a Network Involving Cytochromes P450 and Vitamin D

doi: 10.3390/molecules24081515

Figure Lengend Snippet: Figure 3. Gene expression of the proinflammatory cytokines Il-8 and VEGF-A. The expression of Interleukin 8 (IL-8) and Vascular endothelial growth factor A (VEGF-A) was evaluated at 12 h and 24 h (Panels A and B, and panels C and D, respectively). HFF1 were exposed to ascorbic acid (AA, orange bar), or to 0.5, 0.75 and 1 mg/ml seeds waste extracts (blue bars) or 0.5, 0.75 and 1 mg/mL pulp waste extracts (yellows bar). H2O2 (grey bar) represents HFF1 cells exposed to only H2O2, without previous extracts treatment. The mRNA levels for each gene was expressed as fold of change (2−∆∆Ct) of mRNA levels observed in untreated HFF1 (CTRL, black bar) defined as 1 (mean ± SD; n = 6) and normalized to Glyceraldehyde-3-Phosphate-Dehidrogenase (GAPDH). Data are represented as mean ± SD referred to the control (* p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001).

Article Snippet: Human skin fibroblast 1 (HFF1) cells were purchased from (ATCC, Manassas, VA, USA) and cultured in a basic growing medium of DMEM low glucose (Life Technologies, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (FBS Life Technologies, Carlsbad, California, USA), 2 mM L-glutamine (Euroclone, Milano, Italy) and 1% of penicillin/streptomycin (Euroclone, Milano, Italy).

Techniques: Gene Expression, Expressing, Control

Journal: Molecules

Article Title: Myrtus Polyphenols, from Antioxidants to Anti-Inflammatory Molecules: Exploring a Network Involving Cytochromes P450 and Vitamin D

doi: 10.3390/molecules24081515

Figure Lengend Snippet: Figure 5. CYP27B1 gene expression. The expression of CYP27B1 was evaluated in cells cultured for 12 h and 24 h (Panels A and B) in the presence of different concentrations of pulp and seeds extracts, and then exposed to H2O2. HFF1 were exposed to ascorbic acid (AA, orange bar), or to 0.5, 0.75 and 1 mg/mL seeds waste extracts (blue bars) or 0.5, 0.75 and 1 mg/mL pulp waste extracts (yellows bar). H2O2 (grey bar) represents HFF1 cells exposed to only H2O2, without previous extracts treatment. The mRNA levels for each gene was expressed as fold of change (2yw) of mRNA levels observed in untreated HFF1 (CTRL, black bar) defined as 1 (mean ± SD; n = 6) and normalized to Glyceraldehyde- 3-Phosphate-Dehidrogenase (GAPDH). Data are represented as mean ± SD referred to the control (* p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001).

Article Snippet: Human skin fibroblast 1 (HFF1) cells were purchased from (ATCC, Manassas, VA, USA) and cultured in a basic growing medium of DMEM low glucose (Life Technologies, Carlsbad, CA, USA), supplemented with 10% fetal bovine serum (FBS Life Technologies, Carlsbad, California, USA), 2 mM L-glutamine (Euroclone, Milano, Italy) and 1% of penicillin/streptomycin (Euroclone, Milano, Italy).

Techniques: Gene Expression, Expressing, Cell Culture, Control